Description
Insulin-Transferrin-Selenium (ITS) Supplement contains Insulin, Transferrin, and Sodium Selenite, prepared in Earle’s Balanced Salt Solution (EBSS) without Phenol Red. In addition, ITS-A and ITS-X contain Sodium Pyruvate and Ethanolamine, respectively. ITS supplementation of many conventional synthetic nutrient media permits substantial reduction in the Fetal Bovine Serum (FBS) requirement for routine maintenance and low-density attachment of many adherent cell types.
Contents
Components | Concentration (g/L) | ||
ITS | ITS-A | ITS-X | |
Insulin | 1.00 | 1.00 | 1.00 |
Transferrin | 0.55 | 0.55 | 0.55 |
Sodium Selenite | 0.00067 | 0.00067 | 0.00067 |
Sodium Pyruvate | ----- | 11.00 | ----- |
Ethanolamine | ----- | ----- | 0.20 |
Use
• Each 10 mL vial of Insulin-Transferrin-Selenium 100X Supplement is sufficient for up to one liter of medium.
• In general, it is necessary to add 2–4% FBS to achieve optimal growth, although some adherent cultures may require less serum supplementation following initial adaptation.
• Store Insulin-Transferrin-Selenium supplemented medium in the dark at 2°C to 8°C.
Technical support: 86-19902901483
Order Tel:86-0755-28284050
