Description
Williams' Medium E（WE）was formulated to support long-term cell cultures of adult rat liver epithelial cells, originally developed by Williams and Gunn as reduced serum-supplemented medium. This WE can also be used for growing hepatocyte cells of different species
This WE is a modification of earlier media that has been enriched in amino acids and double the glucose. Otherwise contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate. It contains no proteins or growth factors. Therefore, it requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). WE uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological PH.
This WE is modified with Phenol Red, NaHCO3, Sodium Pyruvate and HEPES, without L-glutamine.
Information
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
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