R&D Products

Description

Insulin-Transferrin-Selenium (ITS) Supplement contains Insulin, Transferrin, and Sodium Selenite, prepared in Earle’s Balanced Salt Solution (EBSS) without Phenol Red. In addition, ITS-A and ITS-X contain Sodium Pyruvate and Ethanolamine, respectively. ITS supplementation of many conventional synthetic nutrient media permits substantial reduction in the Fetal Bovine Serum (FBS) requirement for routine maintenance and low-density attachment of many adherent cell types.

Use 

• Each 10 mL vial of Insulin-Transferrin-Selenium 100X Supplement is sufficient for up to one liter of medium.

• In general, it is necessary to add 2–4% FBS to achieve optimal growth, although some adherent cultures may require less serum supplementation following initial adaptation.

• Store Insulin-Transferrin-Selenium supplemented medium in the dark at 2°C to 8°C.

Contents

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