R&D Products

I Introduction

This product is developed and produced by our company specifically for the recovery process of primary cells. The product can minimize the loss of vitality after cell recovery through testing, greatly improving the recovery efficiency of cells.

II Reagents and Materials

-Recovery medium（Cat#LV-Rec001/002/003）               

- Frozen primary cells

- Crushed ice and ice boxes                                    

- Sterile centrifuge tube of 15 ml

- Wide-mouth pipette tip (The tip of a normal one is cut off and sterilized)  

- Automatic pipette

- Thermostat water bath                                       

-Refrigerated horizontal centrifuge (with horizontal rotor. it is possible to centrifuge 15 ml of centrifuge tubes)

- Biosafety cabinet

III Recovery method (follow the specific instructions for use)

1. Insert the 15 ml of centrifuge tube into an ice box containing enough crushed ice and sterilize it with UV for 15 min. Recovery medium should be sufficiently pre-cooled in crushed ice. Pre-cool the centrifuge at 4 °C and adjust the water bath pot to 38 °C.

2. Quickly transfer the frozen cells from the refrigerated position to thermostat water bath of 38℃. Immerse them in as much water as possible at 38°C and shake it horizontally and clockwise, but make sure the cap remains above the water.

3. Defrost the freezing tube for about 90-120s, until only a small amount of crushed ice floats in it.

4. Disinfect the freezing tube with 75% alcohol and transfer it to a biosafety cabinet.

5. Resuspend the cells with a wide-mouth tip (gently blow 2 times) and transfer to a pre-chilled freezing tube of 15 ml. (The pipet tip and tube can be washed once with about 1 ml of recovery medium).

6. While gently shaking the centrifuge tube of cell suspension, add the recovery medium to the cell suspension dropwise. And add 10 ml of recovery medium to every 1 ml of cell suspension. (Note: When adding, the first 3 ml should be added slowly drop by drop and shaken slightly, and the next 7 ml can be accelerated). Finally, mix slightly upside down once.

Select the appropriate speed and time of centrifugation according to the cell species. Centrifuge at 4 °C, remove the supernatant and resuspend with the corresponding medium before plating the cells into the culture plate for incubation.

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IV Special instructions

This product is free of bacteria, fungi, actinomycetes, mycoplasma and other contaminations, please use with confidence.

Ⅴ Contact Number

Tel:0755-28284050

Technical Support:19902901483 (Dr. Zhou)