R&D Products

I Introduction

Liver sinusoidal endothelial cells (LSEC) play an important role in the occurrence of liver physiological functions and pathological mechanisms, and have become a hot topic in liver research in recent years. It was found that LSEC constitute a semi-permeable barrier between liver parenchymal cells and blood, and are involved in the secretion and regulation of liver metabolism, immune response, growth factors and cytokines in the liver, which play an important role in the physiological function and pathological mechanism of the liver. Liver sinusoidal endothelial cell medium is good at maintaining the cell activity of hepatic sinus endothelial cells and promoting cell proliferation. Note: Add 1% PS to the medium before use.

II Cell culture and passage (human liver sinusoidal endothelial cells as an example)

1. The cells can be passaged when the confluency reaches 80%.

2. Put the medium, PBS and pancreatin into a 38 °C thermostat water bath to preheat. Then wipe with 75% alcohol and put it into the ultra-clean table.

3. Aspirate the old culture solution and add a small amount of PBS to wash the cells. In addition, add an appropriate amount of pancreatin so that the amount of it can cover the cells. Incubate the cells at 37°C for 2-3 min and then can be observed with a microscope.

4. Discard pancreatin when the intercellular space of the adherent cells becomes larger and the cells tend to be rounded but have not yet floated. Then, add fresh medium, shake the cell flask, and terminate the action of pancreatic enzyme. Carefully blow the adherent cells with a pipette to make a cell suspension (control the force of the blowing to avoid generating a large number of bubbles).

5. Centrifuge of 300 × g for 5 min at room temperature. Remove the supernatant and resuspend with medium.

6. Inoculate the cell suspension into a new collagen-coated flask/plate at 3×104cells/cm² and place it in a 37°C/5% CO₂ incubator. The adherent growth of cells can be observed every other day.

III Special Instructions

1. This product is for research use only.

2.   If you find any quality problems, please stop the experiment immediately and contact us:

Tel:0755-28284050

Technical Support:19902901483 (Dr. Zhou)