Product Introduction
Collagen-coated Series
Cat#LV-Coated
(For research use only)
This manual is only applicable to the collagen-coated cell culture plate / dish / bottle made by our company. When you use it, please follow the instructions. If you have any questions, please consult the technical personnel of the company.Ⅰ. Introduction
Type I collagen is an important extracellular matrix that promotes cell apposition, cell differentiation, and maintenance of cell differentiation. Our products are isolated and purified from SD rat tail tendon or pig skin and coated into culture plates under sterile environment. Through strict sterile and quality testing, this product can be used in the cell culture of a variety of primary cells, including hepatocytes, vascular endothelial cells, islet beta cells, etc.
Ⅱ. Reagents and Materials
- Cell culture medium
- Pipette tips
- Automatic pipette
- 75% ethanol disinfectant
- Biosafety cabinets
- 37℃/5% CO 2 incubatorⅢ. Use of Cell Culture Plate
1. Spray 75% ethanol evenly on the outer packaging of collagen-coated plates/dishes/bottles, place in a biosafety cabinet, and remove the packaging.
2. Add sterile PBS per well/vessel/flask in the amount of the corresponding medium used. If a 12-well plate needed, add 1 ml of PBS per well.
3. Shake the coated plate/vessel/flask to wash the remaining acetic acid in it, and remove the PBS to use it.Special instructions: Due to room temperature placement or transportation, collagen-coated plate/vessel /flask may precipitate collagen fibers or salt ion crystals, resulting a "stain" of the plate in the phase contrast microscope. It is normal and does not affect subsequent use.
4. The cell suspension is added to the cell culture plates, vessels, flasks, and the cells are gently shaken out in a 37℃/5% CO 2 incubator.
5. When digesting and passaging, pay special attention to the degree of cell digestion. If the degree of cell digestion is not enough, the addition of serum is likely to reattach the cells, resulting in a low number of cells received.
Suggestion: The cells can be pipetted directly with the digestive solution, which can easily be pipetted after the cells fall. Then add serum-containing medium to terminate the digestion, and quickly pipette the cells down to prevent the cells from re-attaching.
Ⅴ. Customer Service
If you find any quality problems with the products, please collect the original data and contact the company's sales or technical support at the first time. The company will arrange personnel to ensure after-sales. Each laboratory has different conditions, operator habits and proficiency levels. There are objective factors for experimental failure. If the operation is not strictly in accordance with the instructions, exceeding the after-sales time limit, the company will not provide after-sales service. Thank you for your understanding and support.
Validity period and raw data provided:
Group problem: cells cannot group within 48 hours; provide contrast microscope photographs.
Contamination problems: If contamination is found within 96 hours, photographs of phase contrast microscope should be provided.Ⅵ. Contact Number
Tel: 0755-28284050
Technical Support:19902901483 (Dr. Zhou)