Product Introduction
Product
ITS
Catalog
LV-ITS001
Specification
10ml
Free shipping or not
Yes
Delivery
1week
Carriage
LT or RT
Storage
2-8℃
Life cycle
12 months
Warranty
9 months
Insulin,Transferrin,Selenium Solution (ITS),100X
Insulin,Transferrin,Selenium-A Solution (ITS-A),100X
Insulin,Transferrin,Selenium-X Solution (ITS-X),100X
(For Research Use Only. Not for use in diagnostic procedures)
Description
Insulin-Transferrin-Selenium (ITS) Supplement contains Insulin, Transferrin, and Sodium Selenite, prepared in Earle’s Balanced Salt Solution (EBSS) without Phenol Red. In addition, ITS-A and ITS-X contain Sodium Pyruvate and Ethanolamine, respectively. ITS supplementation of many conventional synthetic nutrient media permits substantial reduction in the Fetal Bovine Serum (FBS) requirement for routine maintenance and low density attachment of many adherent cell types.
Safety information
Wear appropriate protective eyewear, clothing, and gloves.
Caution: Human origin materials are non-reactive (donor level) for anti-HIV 1 & 2, anti-HCV, and HBsAg. Handle in accordance with established bio-safety practices.
Use
• Each 10 mL vial of Insulin-Transferrin-Selenium 100X Supplement is sufficient for up to one liter of medium.
• In general, it is necessary to add 2–4% FBS to achieve optimal growth, although some adherent cultures may require less serum supplementation following initial adaptation.
• Store Insulin-Transferrin-Selenium supplemented medium in the dark at 2°C to 8°C.
Product |
Catalog no. |
Amount |
Storage |
Shelf life |
Insulin,Transferrin,Selenium Solution (ITS),100X |
LV-ITS001 |
10ml |
2-8℃ |
12months |
Insulin,Transferrin,Selenium-A Solution (ITS-A),100X |
LV-ITS001A |
|||
Insulin,Transferrin,Selenium-X Solution (ITS-X),100X |
LV-ITS001X |
Contents
Components |
Concentration (g/L) |
||
ITS |
ITS-A |
ITS-X |
|
Insulin |
1.00 |
1.00 |
1.00 |
Transferrin |
0.55 |
0.55 |
0.55 |
Sodium Selenite |
0.00067 |
0.00067 |
0.00067 |
Sodium Pyruvate |
----- |
11.00 |
----- |
Ethanolamine |
----- |
----- |
0.20 |
QC TEST
The results showed that there was no significant difference in the morphology of the primary pig hepatocytes, and the hepatocytes cultured by LV ITS formed a more prominent structure of bile duct.
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