Service Introduction
Service introduction:
Cytotoxicity is a simple cell killing event caused by cells or chemicals, it doesn't depend on the cell death mechanism of apoptosis or necrosis. BALB/c3T3 cells and normal human keratinocytes (NHK) have a good stability and predictability in verification experiments, so we recommended to use general commonly used cell lines for cytotoxicity analysis (another cell line can also be used). The in vitro method helps to predict the systemic and local effects caused by acute chemical exposure and the method also able to assess the toxic concentration in the body. Therefore, before the acute toxicity test, in vitro cytotoxicity analysis should be performed, the LD50 value can be predicted according to the RC prediction model, select the most suitable starting does for acute toxicity in vitro to reduce the laboratory animals’ cost.