Product Introduction
Poly-D-Lysine-coated Cell Culture Plastics
(Cat# LV-PDL coated)
(For scientific research only)
The instruction is only suitable for the Poly-D-Lysine-coated Cell Culture Plastics made by the company. When you use it, please follow the instructions. If you have any questions, please consult the company's technicians.
Ⅰ. Introduction
Liver Biotechnology is a national high-tech enterprise focusing on the isolation, culture, cryopreservation and regeneration of primary hepatocytes. Our products treat the bottom of cell culture plate/vessel/flask with 150-300kDa poly-D-lysine, which can enhance the electrostatic interaction between negatively charged ions on the cell membrane surface and the solid-phase matrix surface to promote cell adherents.
Applicable:
-Enhance the adherent ability of cells by changing the electricity on the surface of the culture plastic.
-Promote cell differentiation and growth of neurites
-Improves the survival rate of primary nerve cell culture
-Culture cells with no serum or low serum
Ⅱ. Reagents and Materials
-Poly-D-Lysine-coated cell culture plate/vessel/flask-cell-culture medium
-Pipet tip
-Automatic pipette
-Thermostatic water bath
-Biosafety cabinet
-37℃/5% CO₂ Incubator
Ⅲ. Pre-processing (this step can be negligible in an emergency case)
1. After sterilizing the outer packaging of poly-D-lysine-coated cell culture plate/vessel/flask with 70-75% alcohol, quickly place it in a biosafety cabinet. Tear the outer packaging to get out the cell culture plate/vessel/flask for later use.
2. UV disinfection for 15 min (in case of emergency, this step can be ignored).
3. Prepare the cultured cell suspension. Add the cell suspension to the pretreated poly-D-lysine cell culture plate/vessel/flask as needed.
Paving density recommended
Primary hepatocytes: 105viable cells/cm2
4. Make up with cell culture solution to the total volume to the recommended amount of culture medium (see Attached Table).
5. Place the culture plate/vessel/flask of the added cells in a 37 °C/5% CO2 incubator. The cells can be adherent within 6-24 hours under normal circumstances.
Addendum: Culture plate/dish/flask with recommended amount of liquid added
Culture plate |
Culture area(cm2) |
Height (with cover mm) |
Recommended amount of culture medium |
6W |
9.5 |
23 |
2mL |
12W |
3.6 |
23 |
1mL |
24W |
1.9 |
23 |
0.5mL |
48W |
0.88 |
23 |
0.3mL |
96/96U/96V |
0.32 |
16 |
0.1mL |
384w |
0.1135 . 11 |
16 |
0.033mL |
35mm |
8.5 |
12 |
2mL |
60mm |
22.9 |
15 |
5mL |
100mm |
57.6 |
20 |
10mL |
150mm |
150.1 |
25 |
25-30mL |
T25 |
25 |
20 |
5mL |
T75 |
75 |
35 |
10mL |
T175 |
175 |
40 |
30mL |
Ⅳ. Customer Service
If you find any quality problems with the products, please collect the original data and contact the company's sales or technical support at the first time. The company will arrange personnel to ensure after-sales. Each laboratory has different conditions, operator habits and proficiency levels. There are objective factors for experimental failure. If the operation is not strictly in accordance with the instructions, exceeding the after-sales time limit, the company will not provide after-sales service. Thank you for your understanding and support.
Validity period and raw data provided:
Group problem: cells cannot group within 48 hours; provide contrast microscope photographs.Contamination problems: If contamination is found within 96 hours, photographs of phase contrast microscope should be provided.
Ⅴ. Contact Number
Tel:0755-28284050
Technical Support:19902901483 (Dr. Zhou)